Uvavanyo lwe-Monkeypox Virus olukhawulezayo lwekiti yovavanyo lwe-2022 MPV Nucleic Acid Detection Kit PCR Test nge-CE Euro Certification

[ INTSHAYELELO ]
*Ikhithi isetyenziselwa ukufumanisa ubulunga be-in vitro iimeko ezikrokrelekayo zeMonkeypox Virus (MPV), iimeko ezidityanisiweyo kunye nezinye iimeko ezifuna ukufunyaniswa ukuba zinosulelo lweMonkeypox Virus.
*Ikhithi isetyenziselwa ukukhangela ijini ye-f3L yeMPV kwimiqala yomqala kunye neesampuli zempumlo.
*Iziphumo zovavanyo zale khithi zezereferensi yeklinikhi kuphela kwaye akufunekanga isetyenziswe njengeyona nqobo yokugweba yoxilongo lwezonyango. Kunconywa ukuba kuqhutywe uhlalutyo olubanzi lwemeko esekelwe kwimibonakaliso yekliniki yesigulane kunye nezinye iimvavanyo zebhubhoratri. 

[ UMBONO WEMVELISO]

[ Umgaqo ]
Le khithi ithatha ulandelelwano oluthile olugciniweyo lwe-MPV f3L yejini njengommandla ekujoliswe kuwo. Itekhnoloji yexesha langempela le-fluorescence quantitative PCR kunye ne-nucleic acid yokukhululwa ngokukhawuleza itekhnoloji isetyenziselwa ukubeka iliso kwi-viral nucleic acid ngokutshintshwa kwesignali ye-fluorescence yeemveliso zokukhulisa. Inkqubo yokufumanisa ibandakanya ukulawulwa kwekhwalithi yangaphakathi, esetyenziselwa ukubeka iliso ukuba ngaba kukho i-PCR inhibitors kwiisampuli okanye ukuba iiseli kwiisampuli zithathwa, ezinokuthi zithintele ngokufanelekileyo imeko embi yobuxoki.

[IZINTO EZIPHAMBILI]

Ikhithi iqulethe ii-reagents zokuqhuba iimvavanyo ezingama-48 okanye ulawulo lomgangatho, kubandakanya la macandelo alandelayo:

Reagent A

Ayinamdla B

Qaphela: Amalungu amanani ebhetshi ahlukeneyo akanakusetyenziswa ngokutshintshanayo

[ Iimeko zoGcino kunye noBomi beShelf ] 
I-1.Reagent A / B inokugcinwa kwi-2-30 ° C, kwaye ubomi beshelufu ziinyanga ezili-10.
2.Nceda uvule ikhava yetyhubhu yovavanyo kuphela xa ulungele uvavanyo.
3.Musa ukusebenzisa iityhubhu zovavanyo ngaphaya komhla wokuphelelwa.
4.Musa ukusebenzisa ityhubhu yokubona evuzayo.
[ Isixhobo Esisebenzayo ]
Ifanelekile kwi-LC480 inkqubo yokuhlalutya kwe-PCR, i-Gentier 48E Inkqubo yohlalutyo lwe-PCR oluzenzekelayo, inkqubo yohlalutyo ye-ABI7500 PCR.

[ IiMfuno zeSampulu ]
1.Iintlobo zeesampuli ezisebenzayo: iisampulu zomqala.
2.Sampling solution: Emva kokuqinisekiswa, kuyacetyiswa ukuba kusetyenziswe i-saline eqhelekileyo okanye ityhubhu yogcino lweNtsholongwane eveliswe yi-Hangzhou Testsea biology ukuqokelela isampulu.
umqala: sula iitoni ze-pharyngeal zamazwe amabini kunye nodonga lwangasemva lwe-pharyngeal kunye ne-swab yesampula engcolileyo elahlayo, cwilisa i-swab kwi-tube equlethe isisombululo se-sampling ye-3mL, ulahle umsila, kwaye uqinise isigqubuthelo sombhobho.
3.Isampuli yokugcina kunye nokuhanjiswa: Iisampulu eziza kuvavanywa kufuneka zivavanywe ngokukhawuleza. Ubushushu bezothutho kufuneka bugcinwe kwi-2 ~ 8ºC. Iisampuli ezinokuvavanywa kwiiyure ezingama-24 zingagcinwa kwi-2ºC ~ 8ºC kwaye ukuba iisampuli azikwazi ukuvavanywa kwiiyure ezingama-24, kufuneka zigcinwe ngaphantsi okanye zilingana ne-70ºC. (ukuba akukho meko yokugcina -70ºC, inokugcinwa kwi -20ºC okwethutyana), kunqande ukuphindaphinda
freezing and thawing.
I-4.I-sampulu echanekileyo yokuqokelela, ukugcinwa, kunye nokuthutha kubaluleke kakhulu ekusebenzeni kwale mveliso.
[Indlela yoVavanyo ]
1.Ukwenziwa kwesampuli kunye nokudibanisa isampuli
1.1 Ukulungiswa kwesampuli
After mixing the above sampling solution with samples, take 30μL of the sample into the DNA release reagent tube and mix it evenly.
1.2 Iyalayisha
Thatha i-20μL ye-reagent yokubuyisela kwaye uyifake kwi-reagent yokufumanisa i-MPV, yongeza i-5μL yesampuli eqhutywe ngasentla (Ulawulo olulungileyo kunye nolawulo olubi luya kucutshungulwa ngokuhambelana neesampuli), vala i-tube cap, i-centrifuge kwi-2000rpm ye-10. imizuzwana.
2. Ukwandiswa kwePCR
2.1 Layisha ipleyiti yePCR elungisiweyo/iityhubhu kwisixhobo sePCR sefluorescence, Ulawulo olubi kunye nolawulo olulungileyo luya kumiselwa kuvavanyo ngalunye.
2.2 Ukusetwa kwetshaneli yeFluorescent:
1)Khetha itshaneli yeFAM yokuchongwa kweMPV;
I-2)Khetha i-HEX / VIC itshaneli yokulawula ufuzo lwangaphakathi;
3.Uhlalutyo lweziphumo
Seta umgca wesiseko ngentla kweyona ndawo iphezulu yegophe le-fluorescent yolawulo olungalunganga.
4.Ulawulo lomgangatho
4.1 Ulawulo olubi: Akukho xabiso le-Ct lifunyenwe kwi-FAM, i-HEX/VIC channel, okanye i-Ct>40;
4.2 Ulawulo olufanelekileyo: Kwi-FAM, i-HEX/VIC channel, Ct≤40;
4.3 Ezi mfuno zingentla kufuneka zaneliswe kuvavanyo olufanayo, kungenjalo iziphumo zovavanyo azivumelekanga kwaye uvavanyo kufuneka luphindwe.
[ Sika ixabiso ]
Isampulu ithathwa njengento entle xa: Ulandelelwano ekujoliswe kulo Ct≤40, Igene yolawulo lwangaphakathi Ct≤40.
[ Iziphumo utoliko ]
Nje ukuba ulawulo lomgangatho lugqithiselwe, abasebenzisi kufuneka bajonge ukuba ngaba kukho ijika lokukhulisa kwisampulu nganye kwitshaneli ye-HEX/VIC, ukuba kukho kunye ne-Ct≤40, ibonise ukuba i-gene yolawulo lwangaphakathi yandisiwe ngempumelelo kwaye olu vavanyo luchanekileyo. Abasebenzisi banokuqhubekeka kuhlalutyo olulandelayo:
3.Kwiisampulu ezinokwandiswa kofuzo lwangaphakathi lolawulo aluphumelelanga (HEX/VIC
itshaneli, Ct> 40, okanye akukho curve yokukhulisa), umthamo weViral ophantsi okanye ubukho be-PCR inhibitor bunokuba sisizathu sokungaphumeleli, uviwo kufuneka luphindwe kwingqokelela yomfanekiso;
4.Iisampulu ezintle kunye nentsholongwane ekhulisiwe, iziphumo zolawulo lwangaphakathi azichaphazeli;
Kwiisampuli ezifunyaniswe zi-negative, ulawulo lwangaphakathi kufuneka luvavanywe ukuba unayo, kungenjalo iziphumo zizonke azikho mthethweni kwaye uvavanyo kufuneka luphindwe, kuqalwa kwinyathelo lokuqokelelwa kwesampula.

[ Ukupakisha kunye nokuThutha ]

[ Ngathi ]