Monkeypox Virus yekukurumidza test kit 2022 MPV Nucleic Acid Detection Kit PCR Test ine CE Euro Certification

[ NHUNGAMIDZO ]
*Kiti iyi inoshandiswa pakuongorora in vitro qualitative yevanofungidzirwa kuti vane utachiona hweMonkeypox Virus (MPV), nyaya dzakaungana uye dzimwe nyaya dzinoda kuonekwa kuti dzine utachiona hweMonkeypox Virus.
*Kiti iyi inoshandiswa kuona f3L gene yeMPV muhuro swabs uye nenasal swab samples.
* Mhedzisiro yebvunzo dzekiti iyi ndeyekuongororwa kwekiriniki chete uye haifanirwe kushandiswa senzira yega yekuongororwa kwekiriniki. Zvinokurudzirwa kuita ongororo yakazara yemamiriro ezvinhu zvichienderana nemurwere kuratidzwa kwekiriniki uye mamwe ma laboratory bvunzo. 

[ PRODUCT FEATURE ]

[ Musimboti ]
Iyi kit inotora iyo chaiyo yakachengetwa kutevedzana kweMPV f3L gene senzvimbo inotariswa. Iyo chaiyo-nguva fluorescence quantitative PCR tekinoroji uye nucleic acid nekukurumidza kuburitsa tekinoroji inoshandiswa kutarisa iyo viral nucleic acid kuburikidza neshanduko yefluorescence chiratidzo chezvigadzirwa zveamplification. Iyo yekuona sisitimu inosanganisira yemukati yemhando yekudzora, iyo inoshandiswa kutarisa kana kune PCR inhibitors mumasampuli kana kuti maseru ari mumasampuri anotorwa, ayo anogona kudzivirira zvinobudirira mamiriro asina kunaka enhema.

[ZVINHU ZVINONYANYA]

Iyo kit ine ma reagents ekugadzirisa makumi mana nemasere bvunzo kana kudzora kwemhando, kusanganisira zvinotevera zvikamu:

Reagent A

Reagent B

Ongorora: Izvo zvikamu zvenhamba dzakasiyana dzebatch hazvigone kushandiswa zvakasiyana

[ Mamiriro Ekuchengetedza Uye Hupenyu Hwesherufu] 
1.Reagent A / B inogona kuchengetwa pa 2-30 ° C, uye hupenyu hwesherufu ndeye 10 mwedzi.
2.Ndapota vhura test tube cover chete kana wagadzirira bvunzo.
3.Usashandisa test tubes kupfuura zuva rekupera.
4.Usashandisa chubhu yekuona inodonha.
[ Chishandiso Chinoshanda]
Inokodzera LC480 PCR yekuongorora system, Gentier 48E Automatic PCR yekuongorora system, ABI7500 PCR yekuongorora system.

[Sample Zvinodiwa]
1.Inoshanda mhando dzemhando: throat swabs samples.
2.Sampling mhinduro: Mushure mekuona, zvinokurudzirwa kushandisa yakajairika saline kana Virus kuchengetedza chubhu inogadzirwa neHangzhou Testsea biology yekuunganidza sampuli.
swab pahuro: pukuta bilateral pharyngeal tonsils uye posterior pharyngeal madziro nekuraswa sterile sampling swab, nyudza swab muchubhu ine 3mL sampling solution, kurasa muswe, uye sunga chubhu chivharo.
3.Sample kuchengetedza uye kutumira: Mienzaniso inofanirwa kuongororwa inofanirwa kuongororwa nekukurumidza sezvinobvira. Tembiricha yekufambisa inofanirwa kuchengetwa pa2 ~ 8ºC. Masampuli anogona kuongororwa mukati meawa makumi maviri nemana anogona kuchengetwa pa24ºC ~ 2ºC uye kana masampuli asingakwanisi kuongororwa mukati meawa makumi maviri nemana, anofanira kuchengetwa ari pasi pe kana kuenzana -8ºC. (kana pasina kuchengetedza kwe -24ºC, inogona kuchengetwa pa -70ºC kwenguva pfupi), dzivisa kudzokororwa
kutonhora nekunyunguduka.
4.Kutora sampuli yakakodzera, kuchengetedza, uye kutakura zvakakosha pakushanda kwechigadzirwa ichi.
[Kuedza Nzira]
1.Sample processing and sample addition
1.1 Kuenzanisira
Mushure mekusanganisa mhinduro yesampling iri pamusoro nemasample, tora 30μL yemuenzaniso muDNA inoburitsa reagent chubhu wosanganisa zvakaenzana.
1.2 Loading
Take 20μL of the reconstitution reagent and add it to the MPV detection reagent, add 5μL of the above processed sample (The positive control and negative control shall be processed in parallel with the samples), cover the tube cap, centrifuge it at 2000rpm for 10 seconds.
2. PCR kuwedzera
2.1 Takura iyo yakagadzirirwa PCR ndiro / machubhu kune fluorescence PCR chiridzwa, Negative control uye kutonga kwakanaka kuchaiswa kune yega bvunzo.
2.2 Fluorescent chiteshi kuseta:
1)Sarudza FAM chiteshi chekuonekwa kweMPV;
2)Sarudza HEX/VIC chiteshi chemukati chekudzora gene kuona;
3.Kuongorora kwemigumisiro
Gadzirisa mutsara wepasi pamusoro penzvimbo yepamusoro yeiyo negative control’s fluorescent curve.
4.Quality control
4.1 Negative control: Hapana Ct value yakawanikwa muFAM, HEX/VIC chiteshi, kana Ct> 40;
4.2 Kudzora kwakanaka: MuFAM,HEX/VIC chiteshi, Ct≤40;
4.3 Zvinodiwa zviri pamusoro zvinofanirwa kugutsikana mukuyedza kumwe chete, zvikasadaro mhedzisiro yebvunzo haina basa uye kuedza kunoda kudzokororwa.
[Kubvisa kukosha]
Sample inoonekwa seyakanaka kana: Target sequence Ct≤40, The internal control gene Ct≤40.
[ Results interpretation ]
Kana kutonga kwemhando kwapfuura, vashandisi vanofanirwa kutarisa kana paine amplification curve yesample yega yega muHEX/VIC chiteshi, kana iripo uye neCt≤40, yakaratidza kuti yemukati yekudzora gene yakakwidziridzwa zvinobudirira uye iyi bvunzo inoshanda. Vashandisi vanogona kuenderera kune yekutevera ongororo:
3.Kumasamples ane kukwidziridzwa kwegene rekutonga kwemukati kwakakundikana (HEX/VIC
chiteshi, Ct> 40, kana pasina amplification curve), yakaderera Viral load kana kuvepo kwePCR inhibitor inogona kunge iri chikonzero chekutadza, bvunzo dzinofanira kudzokororwa kubva mukuunganidzwa kwemuenzaniso;
4.Kune sampuli dzakanaka uye hutachiwana hwakagadzirwa, migumisiro yekutonga kwemukati haibatsiri;
Kune masampuli akaongororwa kuti haana, kutonga kwemukati kunoda kuongororwa kuti ane positive zvikasadaro mhedzisiro yacho haina kunaka uye bvunzo inoda kudzokororwa, kutanga kubva padanho rekuunganidza.

[ Kuisa & Kutakura ]

[ Nezvedu ]