Ngwa nnwale ngwa ngwa nke ọrịa Monkeypox 2022 MPV Nucleic Acid Detection Kit PCR Test with CE Euro Certification

[ NKWUO:
*A na-eji ngwa ahụ maka nchọpụta in vitro qualitative nke ndị a na-enyo enyo na ha bụ Nje Virus Monkeypox (MPV), clustered cases na ndị ọzọ * kwesịrị ịchọpụta maka ọrịa Monkeypox.
* A na-eji ngwa ahụ chọpụta mkpụrụ ndụ f3L nke MPV n’ime akpịrị akpịrị na ihe nlele imi.
*Nchọpụta ule nke kit a bụ maka naanị nrụtụ aka n’ụlọọgwụ ma ekwesighi iji ya dịka naanị akara maka nyocha ụlọ ọgwụ. A na-atụ aro ka ịme nyocha zuru oke nke ọnọdụ ahụ dabere na ngosipụta ụlọ ọgwụ nke onye ọrịa na nyocha ụlọ nyocha ndị ọzọ. 

[Ụkpụrụ ngwaahịa]

[Iwu]
Ngwa a na-ewere usoro echekwara nke MPV f3L dị ka mpaghara ebumnuche. A na-eji teknụzụ PCR nke ezigbo fluorescence quantitative na teknụzụ ntọhapụ ngwa ngwa nucleic acid iji nyochaa nje nucleic acid site na mgbanwe nke fluorescence mgbaàmà nke ngwaahịa mmụba. Usoro nchọpụta na-agụnye njikwa mma nke ime, nke a na-eji nyochaa ma enwere PCR inhibitors na nlele ma ọ bụ na sel ndị dị na ihe nlele ahụ na-ewere ya, nke nwere ike igbochi ọnọdụ ọjọọ ụgha nke ọma.

[Isi ihe mejupụtara]

The kit contains reagents for processing 48 tests or quality control, including the following components:

Reagent A

Reag B

Mara: Enweghị ike iji ihe mejupụtara ọnụọgụ ọnụọgụ dị iche iche

[ Ọnọdụ Nchekwa na Ndụ Shelf ] 
1.Reagent A / B nwere ike ịchekwa na 2-30 ° C, na ndụ shelf bụ ọnwa 10.
2.Please open the test tube cover only when you are ready for the test.
3.Una eji tubes ule gafere ụbọchị njedebe.
4.Do not use a leaking detection tube.
[ Applicable Instrument ]
Kwesịrị ekwesị maka usoro nyocha LC480 PCR, Gentier 48E Automatic PCR analysis system, ABI7500 PCR analysis system.

[Ihe atụ chọrọ]
1.Applicable sample types: akpịrị swabs sample.
2.Sampling ngwọta: Mgbe nyochachara, a na-atụ aro ka iji saline nkịtị ma ọ bụ tube nchekwa nje nke Hangzhou Testsea biology mepụtara maka nchịkọta nlele.
akpịrị akpịrị: wipe bilateral pharyngeal tonsils and posterior pharyngeal wall with disposable sterile sampling swab, immerse the swab into the tube containing 3mL sampling solution, discard the tail, and tighten the tube cover.
3.Sample nchekwa na nnyefe: Ekwesịrị ịnwale ihe nlele a ga-anwale ozugbo enwere ike. A ga-edebe okpomọkụ njem njem na 2 ~ 8ºC. A ga-echekwa ihe nlele nke a ga-anwale n’ime awa 24 na 2ºC ~ 8ºC ma ọ bụrụ na enweghị ike ịnwale ihe nlele n’ime awa 24, a ga-echekwa ya na ihe na-erughị ma ọ bụ hà -70ºC. (ọ bụrụ na enweghị ọnọdụ nchekwa nke -70ºC, enwere ike ịchekwa ya na -20ºC nwa oge), zere ugboro ugboro.
kefriza na thawing.
4.Nchịkọta ihe atụ kwesịrị ekwesị, nchekwa, na njem dị oké mkpa maka ịrụ ọrụ nke ngwaahịa a.
[Ụzọ nnwale]
1.Sample nhazi na ntinye ihe atụ
1.1 Nlele nlele
Mgbe agwakọta ihe ngwọta nlele dị n’elu na ihe nlele, were 30μL nke nlele ahụ n’ime tube reagent nke DNA ma gwakọta ya nke ọma.
1.2 na-ebu
Were 20μL nke reconstitution reagent ma tinye ya na MPV nchọpụta reagent, gbakwunye 5μL nke n’elu esichara sample (The nti njikwa na-adịghị mma akara ga-esichara na yiri na samples), kpuchie tube okpu, centrifuge ya na 2000rpm maka 10. sekọnd.
2. PCR nkwalite
2.1 Ibu Ibu PCR efere / tubes a kwadebere na ngwa PCR fluorescence, njikwa na-adịghị mma na njikwa dị mma ga-edozi maka ule ọ bụla.
2.2 Ntọala ọwa fluorescent:
1) Họrọ ọwa FAM maka nchọpụta MPV;
2) Họrọ ọwa HEX / VIC maka nchọpụta mkpụrụ ndụ n’ime;
3.Ntụle nsonaazụ
Tọọ ahịrị ntọala n’elu ebe kachasị elu nke mgbanaka fluorescent nke njikwa adịghị mma.
4. Quality njikwa
4.1 Negative control:No Ct value detected in FAM,HEX/VIC channel, or Ct>40;
4.2 njikwa dị mma: Na FAM, HEX / VIC ọwa, Ct≤40;
4.3 Ihe ndị a chọrọ n’elu kwesịrị inwe afọ ojuju n’otu nnwale ahụ, ma ọ bụghị ya, nsonaazụ ule adịghị mma na nnwale ahụ kwesịrị ka emeghachi ya.
[Bepụ uru]
A na-ewere nlele anya dị ka ihe dị mma mgbe: Usoro ebumnuche Ct≤40, mkpụrụ ndụ njikwa ime Ct≤40.
[ Results interpretation ]
Ozugbo agafere njikwa mma, ndị ọrụ kwesịrị ịlele ma ọ bụrụ na enwere mgbatị mmụba maka nlele ọ bụla na ọwa HEX / VIC, ọ bụrụ na enwere yana yana Ct≤40, ọ na-egosi na mkpụrụ ndụ njikwa nke ime na-abawanye nke ọma na nnwale a dị irè. Ndị ọrụ nwere ike ịga n’ihu na nyocha ndị a:
3.For samples na amplification nke esịtidem akara gene dara (HEX / VIC
channel, Ct>40,or no amplification curve), low Viral load or the existence of PCR inhibitor could be the reason of failure, the examination should repeated from the specimen collection;
4.For nti samples na omenala nje, pụta nke esịtidem akara adịghị emetụta;
Maka ihe nlele nwalere adịghị mma, ekwesịrị ịnwale njikwa ime ahụ nke ọma ma ọ bụghị ya n’ozuzu ya abaghị uru ma ọ dị mkpa ka emeghachi nyocha ahụ, malite na usoro nchịkọta ihe atụ.

[ Mbukota & ationgbọ njem ]

[ Gbasara anyị ]