Xirmada Busbuska Virus-ka degdega ah 2022 MPV Qalabka ogaanshaha Acid Nucleic Acid PCR oo wata shahaadada CE Euro

[ HORDHAC ]
*Qalabka waxa loo isticmaalaa in vitro lagu ogaado tayooyinka laga shakiyo ee Fayraska Monkeybux (MPV), kiisas urursan iyo xaalado kale oo * u baahan in laga ogaado caabuqa Fayraska Monkeybux.
*Qalabka waxa loo isticmaalaa in lagu ogaado hiddasidaha f3L ee MPV ee suufka cunaha iyo muunadaha suufka sanka.
*Natiijooyinka baaritaanka xirmadan waxaa loogu talagalay tixraaca bukaan-socodka oo keliya mana aha in loo istcimaalo sidii halbeegga keliya ee ogaanshaha bukaan-socodka. Waxaa lagu talinayaa in la sameeyo falanqayn dhamaystiran oo ku saabsan xaaladda ku salaysan calaamadaha bukaan-socodka iyo shaybaarka kale ee shaybaarka. 

[ MUUQAALKA WAX soo saarka

[ Mabda ‘
Qalabkani waxa uu qaadanayaa taxanaha gaarka ah ee la dhawray ee hidda-wadaha MPV f3L sidii gobolka la beegsanayo. Tiknoolajiyada PCR ee wakhtiga-dhabta ah ee fluorescence-ga iyo tignoolajiyada siidaynta degdega ah ee aashitada nucleic ayaa loo isticmaalaa si loola socdo fayraska nucleic acid iyada oo loo marayo isbeddelka calaamada fluorescence ee alaabada kor u qaadida. Nidaamka ogaanshaha waxaa ka mid ah ilaalinta tayada gudaha, kaas oo loo isticmaalo in lagu kormeero haddii ay jiraan PCR inhibitors shaybaarada ama haddii unugyada shaybaarada la qaaday, taas oo si wax ku ool ah uga hortagi karta xaaladda xun ee beenta ah.

[XAALADAHA GUUD]

Xirmada ayaa ka kooban reagenyaal lagu farsameeyo 48 tijaabo ama ilaalinta tayada, oo ay ku jiraan qaybaha soo socda:

Reagent A

Reagent B

Xusuusin: Qaybaha nambarada dufcadaha kala duwan looma isticmaali karo si isku beddelasho ah

[ Xaaladaha Kaydinta iyo Nolosha shelf ] 
1.Reagent A/B can be stored at 2-30°C, and the shelf life is 10 months.
2.Please open the test tube cover only when you are ready for the test.
3.Ha isticmaalin tuubooyinka tijaabada wixii ka dambeeya taariikhda uu dhacayo.
4.Ha isticmaalin tuubada ogaanshaha ee soo daadanaya.
[Qalabka lagu dabaqi karo]
Suitable for Suitable for LC480 PCR analysis system, Gentier 48E Automatic PCR analysis system, ABI7500 PCR analysis system.

[ Sample Requirements ]
1.Applicable sample types: muunadaha suufka cunaha.
2.Sampling xal: After verification, it is recommended to use normal saline or Virus preservation tube produced by Hangzhou Testsea biology for sample collection.
throat swab: Ku masax qumanka qanjidhada laba geesoodka ah iyo gidaarka xuubka dambe ee suufka muunad la tuuri karo, geli suufka tuubada ka kooban 3ml muunad, tuur dabada, oo adkee daboolka tuubada.
3.Sample kaydinta iyo keenista: The samples to be tested should be tested as soon as possible. The transportation temperature should be kept at 2~8ºC.The samples that can be tested within 24 hours can be stored at 2ºC~8ºC and if the samples cannot be tested within 24 hours, it should be stored at less than or equal to -70ºC (if there is no storage condition of -70ºC, it can be stored at -20ºC temporarily), avoid repeated
freezing and thawing.
4.Proper sample collection, storage, and transportation are critical to the performance of this product.
[Habka Tijaabada]
1.Sample processing iyo muunad ku darida
1.1 Sambal samaynta
Ka dib marka lagu qaso muunada sare ee muunado, qaado 30μL oo muunada ah tuubada reagent ee DNA oo si siman isku walaaq.
1.2 Soodejinaya
Take 20μL of the reconstitution reagent and add it to the MPV detection reagent, add 5μL of the above processed sample (The positive control and negative control shall be processed in parallel with the samples), cover the tube cap, centrifuge it at 2000rpm for 10 seconds.
2. Kordhinta PCR
2.1 Ku shub saxanka PCR/tubooyinka diyaarsan ee qalabka PCR-ga ee fluorescence, xakamaynta xun iyo xakamaynta togan waa in loo dejiyaa imtixaan kasta.
2.2 Fluorescent channel setting:
1)Choose FAM channel for MPV detection;
2) Dooro kanaalka HEX / VIC ee ogaanshaha hidda-socodka kontoroolka gudaha;
3.Results analysis
Set the base line above the highest point of the negative control’s fluorescent curve.
4.Quality control
4.1 Xakamaynta taban: Wax qiimo ah oo Ct ah lagama helin FAM, kanaalka HEX/VIC, ama Ct> 40;
4.2 Positive control:In FAM,HEX/VIC channel, Ct≤40;
4.3 Shuruudaha kor ku xusan waa in lagu qanco isla tijaabadaas, haddii kale natiijadii imtixaanku waa mid khaldan oo tijaabada ayaa loo baahan yahay in lagu celiyo.
[Goy qiimaha]
A sample is considered as positive when: Target sequence Ct≤40, The internal control gene Ct≤40.
[ Fasiraadda Natiijooyinka ]
Marka xakamaynta tayada la dhaafo, isticmaalayaashu waa inay hubiyaan haddii uu jiro qalooca xoojinta muunad kasta oo ku yaal kanaalka HEX/VIC, haddii uu jiro iyo Ct≤40, waxay muujisay hidda-wadaha kontoroolka gudaha ayaa si guul leh loo xoojiyay tijaabadan gaarka ahna waa ansax. Isticmaalayaashu waxay sii wadi karaan falanqaynta dabagalka:
3.Samballada leh kordhinta hidda-socodka xakamaynta gudaha ayaa fashilmay (HEX/VIC
kanaalka, Ct> 40, ama qalooca kor u qaadis la’aanta), culeyska fayraska hooseeya ama jiritaanka PCR inhibitor waxay noqon kartaa sababta guul dareysiga, baaritaanka waa in lagu celiyaa ururinta muunada;
4.Samballada togan iyo fayraska dhaqameed, natiijooyinka xakamaynta gudaha ma saameeyaan;
Shaybaarada lagu tijaabiyay diidmada, kontoroolka guduhu wuxuu u baahan yahay in la tijaabiyo si togan haddii kale natiijadu guud ahaan waa buray, baadhitaankuna wuxuu u baahan yahay in lagu celiyo, laga bilaabo tilaabada ururinta muunada.

[ Xirxirida & Gaadiidka ]

[ Nagu saabsan ]