Monkeypox Virus sai test kit 2022 MPV Nucleic Acid Detection Kit PCR Test nrog CE Euro Certification

[ INTRODUCTION ]
* Cov khoom siv yog siv rau hauv vitro zoo nrhiav pom cov neeg xav tias muaj tus kab mob Monkeypox Virus (MPV), pawg neeg mob thiab lwm yam mob uas * yuav tsum tau kuaj pom tus kab mob Monkeypox Virus.
*The kit is used to detect the f3L gene of the MPV in throat swabs and nasal swab samples.
*The test results of this kit are for clinical reference only and should not be used as the sole criterion for clinical diagnosis. It is recommended to conduct a comprehensive analysis of the condition based on the patient’s clinical manifestations and other laboratory tests. 

[ PRODUCT FEATURE ]

[ Principle ]
Cov khoom siv no siv cov kev khaws cia tshwj xeeb ntawm MPV f3L gene raws li thaj tsam lub hom phiaj. Lub sijhawm tiag tiag fluorescence quantitative PCR thev naus laus zis thiab nucleic acid sai tso cov thev naus laus zis yog siv los saib xyuas cov kab mob nucleic acid los ntawm kev hloov pauv ntawm fluorescence teeb liab ntawm cov khoom lag luam amplification. Cov txheej txheem kuaj pom muaj xws li kev tswj hwm sab hauv, uas yog siv los saib xyuas seb puas muaj PCR inhibitors hauv cov qauv lossis seb cov hlwb hauv cov qauv raug coj mus, uas tuaj yeem tiv thaiv qhov xwm txheej tsis zoo.

[MAIN KEV CWJ PWM]

Cov khoom siv muaj cov tshuaj reagents rau kev ua 48 qhov kev xeem lossis kev tswj xyuas zoo, suav nrog cov khoom hauv qab no:

Reagent A

Tseem Tshuav B

Note: The components of different batch numbers cannot be used interchangeably

[Storage Conditions Thiab Txee Lub Neej] 
1.Reagent A / B tuaj yeem khaws cia ntawm 2-30 ° C, thiab lub txee lub neej yog 10 lub hlis.
2.Please open the test tube cover only when you are ready for the test.
3.Tsis txhob siv cov raj xa mus dhau hnub tas sij hawm.
4.Tsis txhob siv lub raj xa dej kom pom.
[Cov cuab yeej siv tau]
Tsim nyog rau LC480 PCR tsom xam qhov system, Gentier 48E Tsis Siv Neeg PCR tsom xam qhov system, ABI7500 PCR tsom xam qhov system.

[Sample Requirement]
1.Siv hom qauv: caj pas swabs kuaj.
2.Sampling tov: After verification, it is recommended to use normal saline or Virus preservation tube produced by Hangzhou Testsea biology for sample collection.
throat swab: so ob sab pharyngeal tonsils thiab posterior pharyngeal phab ntsa nrog pov tseg sterile sampling swab, raus lub swab rau hauv lub raj uas muaj 3mL sampling tov, pov tseg tus Tsov tus tw, thiab zawm lub raj npog.
3.Sample cia thiab xa khoom: Cov qauv yuav tsum tau kuaj kom sai li sai tau. Kev thauj mus los kub yuav tsum tau khaws cia ntawm 2 ~ 8ºC.Cov qauv uas tuaj yeem kuaj tau hauv 24 teev tuaj yeem khaws cia ntawm 2ºC ~ 8ºC thiab yog tias cov qauv tsis tuaj yeem kuaj hauv 24 teev, nws yuav tsum khaws cia tsawg dua lossis sib npaug ntawm -70ºC (yog tias tsis muaj qhov chaw cia ntawm -70ºC, nws tuaj yeem khaws cia ntawm -20ºC ib ntus), tsis txhob rov ua dua
khov thiab thawing.
4.Proper sample collection, storage, and transportation are critical to the performance of this product.
[Testing Method ]
1.Sample processing thiab coj mus kuaj ntxiv
1.1 Qauv coj los ua
Tom qab sib tov cov qauv saum toj no nrog cov qauv, coj 30μL ntawm cov qauv mus rau hauv DNA tso reagent raj thiab sib tov sib npaug.
1.2 Chaw thau khoom
Take 20μL of the reconstitution reagent and add it to the MPV detection reagent, add 5μL of the above processed sample (The positive control and negative control shall be processed in parallel with the samples), cover the tube cap, centrifuge it at 2000rpm for 10 seconds.
2. PCR amplification
2.1 Thauj khoom PCR phaj / raj rau lub fluorescence PCR ntsuas, tsis zoo tswj thiab tswj zoo yuav tsum teem rau txhua qhov kev xeem.
2.2 Fluorescent channel teeb tsa:
1) Xaiv FAM channel rau MPV nrhiav pom;
2)Choose HEX/VIC channel for internal control gene detection;
3. Cov txiaj ntsig kev txheeb xyuas
Teem lub hauv paus kab saum toj ntawm qhov siab tshaj plaws ntawm kev tswj tsis zoo ntawm fluorescent nkhaus.
4.Quality control
4.1 Kev tswj tsis zoo: Tsis muaj tus nqi Ct kuaj pom hauv FAM, HEX / VIC channel, lossis Ct> 40;
4.2 Kev tswj zoo: Hauv FAM, HEX / VIC channel, Ct≤40;
4.3 Cov kev cai saum toj no yuav tsum txaus siab rau tib qhov kev sim, txwv tsis pub cov txiaj ntsig kev xeem tsis raug thiab qhov kev sim yuav tsum tau rov ua dua.
[ Txiav tawm tus nqi ]
Ib qho piv txwv raug suav tias yog qhov zoo thaum: Lub Hom Phiaj Ct≤40, Cov Kev Tswj Sab Hauv Ct≤40.
[Kev txhais cov ntsiab lus]
Thaum qhov kev tswj xyuas zoo dhau lawm, cov neeg siv yuav tsum xyuas seb puas muaj qhov kev nthuav dav dav rau txhua tus qauv hauv HEX / VIC channel, yog tias muaj thiab nrog Ct≤40, nws tau qhia tias cov noob hauv cov tswj tau ua tiav thiab qhov kev sim tshwj xeeb no siv tau. Cov neeg siv tuaj yeem mus rau qhov kev soj ntsuam tom qab:
3.For samples with the amplification of internal control gene failed (HEX/VIC
channel, Ct>40,or no amplification curve), low Viral load or the existence of PCR inhibitor could be the reason of failure, the examination should repeated from the specimen collection;
4.Rau cov qauv zoo thiab kab lis kev cai, cov txiaj ntsig ntawm kev tswj hwm sab hauv tsis cuam tshuam;
Rau cov qauv kuaj pom qhov tsis zoo, kev tswj xyuas sab hauv yuav tsum tau kuaj pom qhov zoo yog tias tag nrho cov txiaj ntsig tsis raug thiab qhov kev ntsuam xyuas yuav tsum tau rov ua dua, pib los ntawm cov kauj ruam sau cov qauv.

[ Ntim & Thauj ]

[ Hais txog peb ]