Monkeypox Virus ea tlhahlobo e potlakileng ea 2022 MPV Nucleic Acid Detection Kit PCR Test with CE Euro Certification

[ SELELEKELA ]
*Sephutheloana se sebelisoa bakeng sa tlhahlobo ea in vitro ea maemo a belaelloang a Monkeypox Virus (MPV), linyeoe tse kopaneng le linyeoe tse ling tse hlokang ho hlahlojoa hore li na le tšoaetso ea Monkeypox Virus.
*Thepa e sebelisoa ho bona mofuta oa f3L oa MPV ka har’a ‘metso le lisampole tsa nasal swab.
*The test results of this kit are for clinical reference only and should not be used as the sole criterion for clinical diagnosis. It is recommended to conduct a comprehensive analysis of the condition based on the patient’s clinical manifestations and other laboratory tests. 

[ SEHLAHAKA SEHLOOHO ]

[ Molao-motheo ]
Setsi sena se nka tatellano e bolokehileng ea mofuta oa MPV f3L joalo ka sebaka seo ho shebiloeng ho sona. Theknoloji ea nako ea ‘nete ea fluorescence quantitative PCR le thekenoloji ea “nucleic acid” e potlakileng e sebelisoa ho lekola viral nucleic acid ka phetoho ea lets’oao la fluorescence la lihlahisoa tsa amplification. Mokhoa oa ho lemoha o kenyelletsa taolo ea boleng ba ka hare, e sebelisetsoang ho hlahloba hore na ho na le PCR inhibitors ka lisampole kapa hore na lisele tsa lisampole li nkiloe, tse ka thibelang ka katleho boemo bo bobe ba bohata.

[LIEKETSENG SEHOLO]

Thepa e na le li-reagents bakeng sa ho sebetsana le liteko tse 48 kapa taolo ea boleng, ho kenyelletsa le likarolo tse latelang:

Reagent A

Reagent B

Tlhokomeliso: Likarolo tsa linomoro tse fapaneng tsa batch li ke ke tsa sebelisoa ka ho fapanyetsana

[ Maemo a Polokelo le Bophelo ba Shelf] 
1.Reagent A / B e ka bolokoa ho 2-30 ° C, ‘me bophelo ba sethala ke likhoeli tse 10.
2.Ka kopo bula sekoahelo sa tube ea teko feela ha u se u itokiselitse tlhahlobo.
3.U se ke ua sebelisa li-tubes tsa teko ho feta letsatsi la ho fela.
4.Do not use a leaking detection tube.
[Sesebediswa se Sebeletsang]
E loketse bakeng sa Sistimi ea tlhahlobo ea LC480 PCR, sistimi ea tlhahlobo ea Gentier 48E Automatic PCR, sistimi ea tlhahlobo ea ABI7500 PCR.

[ Litlhoko tsa Mohlala ]
1.Mefuta ea mehlala e sebetsang: lisampole tsa ‘metso.
2. Sampling tharollo: Kamora ho netefatsoa, ​​​​ho khothaletsoa ho sebelisa tšupa e tloaelehileng ea letsoai kapa kokoana-hloko e hlahisoang ke Hangzhou Testsea biology bakeng sa ho bokella sampole.
mometso: Hlakola lithane tse peli tsa pharyngeal le lebota le ka morao la pharyngeal ka swab ea sampole e hloekisitsoeng, kenya sesepa ka har’a tube e nang le 3mL sampling solution, u lahle mohatla, ‘me u tiise sekoaelo sa tube.
3.Sample polokelo le delibari: Mehlala e tla hlahlojoa e lokela ho hlahlojoa kapele kamoo ho ka khonehang. Thempereichara ea lipalangoang e lokela ho bolokoa ho 2 ~ 8ºC. Mehlala e ka hlahlojoang nakong ea lihora tse 24 e ka bolokoa ho 2ºC ~ 8ºC mme haeba lisampole li ke ke tsa lekoa nakong ea lihora tse 24, li lokela ho bolokoa ka tlase ho kapa ho lekana le -70ºC. (haeba ho se na boemo ba polokelo ea -70ºC, e ka bolokoa ho -20ºC ka nakoana), qoba ho pheta-pheta
freezing and thawing.
4.Proper sample collection, storage, and transportation are critical to the performance of this product.
[Mokhoa oa ho Lekola]
1.Sample processing le ho eketsa mohlala
1.1 Mohlala oa ts’ebetso
Kamora ho kopanya tharollo ea sampole e kaholimo le lisampole, nka 30μL ea sampole ka har’a tube ea reagent ea DNA ebe u e kopanya ka ho lekana.
1.2 Ho kenya
Nka 20μL ea reagent ea ho tsosolosa ‘me u e kenye mochineng oa ho lemoha oa MPV, eketsa 5μL ea sampuli e entsoeng ka holimo (Taolo e ntle le taolo e mpe e tla sebetsoa ka ho tsamaisana le lisampole), koahela cap cap, e centrifuge ka 2000rpm bakeng sa 10. metsotsoana.
2. Matlafatso ea PCR
2.1 Laola poleiti ea PCR e lokiselitsoeng ho sesebelisoa sa PCR sa fluorescence, Taolo e mpe le taolo e nepahetseng e tla beoa tekong ka ‘ngoe.
2.2 Fluorescent channel setting:
1) Khetha mocha oa FAM bakeng sa ho lemoha MPV;
2) Khetha mocha oa HEX / VIC bakeng sa ho lemoha liphatsa tsa lefutso tsa ka hare;
3.Tlhahlobo ea liphetho
Beha mola oa motheo ka holim’a ntlha e phahameng ka ho fetisisa ea mothinya oa fluorescent oa taolo e mpe.
4.Ho laola boleng
4.1 Taolo e fosahetseng: Ha ho boleng ba Ct bo fumanoeng ho FAM, HEX/VIC channel, kapa Ct> 40;
4.2 Taolo e ntle: Ka mocha oa FAM, HEX / VIC, Ct≤40;
4.3 Litlhoko tse ka holimo li lokela ho khotsofatsoa tekong e tšoanang, ho seng joalo liphetho tsa liteko li fosahetse ‘me teko e lokela ho phetoa.
[ Fetoha boleng ]
A sample is considered as positive when: Target sequence Ct≤40, The internal control gene Ct≤40.
[ Results interpretation ]
Hang ha taolo ea boleng e fetisitsoe, basebelisi ba lokela ho hlahloba hore na ho na le curve ea amplification bakeng sa sampole ka ‘ngoe ho kanale ea HEX/VIC, haeba e le teng, ‘me ka Ct≤40, e bontšitse hore mofuta oa taolo ea kahare o matlafalitsoe ka katleho mme tlhahlobo ena e nepahetse. Basebelisi ba ka tsoela pele ho etsa tlhahlobo e latelang:
3. Bakeng sa disampole tse nang le amplification ea gene ea taolo ea ka hare e hlōlehile (HEX/VIC
channel, Ct>40, kapa no amplification curve), low Viral load kapa ho ba teng ha PCR inhibitor e ka ba lebaka la ho hlōleha, tlhahlobo e lokela ho phetoa ho tsoa pokellong ea mohlala;
4.Bakeng sa disampole tse ntle le kokoana-hloko e hlahisitsoeng, liphello tsa taolo ea ka hare ha li ame;
Bakeng sa disampole tse fumanweng di se na HIV, taolo ya ka hare e lokela ho lekolwa e le ntle, ho seng jwalo sephetho se fosahetse, mme tlhahlobo e lokela ho phetwa, ho qala ka mohato wa ho bokella mohlala.

[ Paka & Lipalangoang ]

[ Mabapi le rona ]