Monkeypox virusi tezkor sinov to’plami 2022 MPV nuklein kislotasini aniqlash to’plami Idoralar evro sertifikati bilan PCR testi

[ KIRISH ]
*To’plam maymunchechak virusi (MPV) bilan kasallangan, klasterlangan holatlar va *maymunchechak virusi infektsiyasiga tashxis qo’yilishi kerak bo’lgan boshqa holatlarni in vitro sifatli aniqlash uchun ishlatiladi.
*Toʻplam tomoq va burun tampon namunalarida MPV ning f3L genini aniqlash uchun ishlatiladi.
*Ushbu to’plamning sinov natijalari faqat klinik ma’lumot uchun mo’ljallangan va klinik tashxis uchun yagona mezon sifatida foydalanilmasligi kerak. Bemorning klinik ko’rinishlari va boshqa laboratoriya tekshiruvlari asosida vaziyatni har tomonlama tahlil qilish tavsiya etiladi. 

[ PRODUCT FEATURE ]

[Prinsip]
Ushbu to’plam maqsadli hudud sifatida MPV f3L genining o’ziga xos saqlanib qolgan ketma-ketligini oladi. Haqiqiy vaqtda floresan miqdoriy PCR texnologiyasi va nuklein kislotani tez chiqarish texnologiyasi kuchaytiruvchi mahsulotlarning floresan signalini o’zgartirish orqali virusli nuklein kislotani kuzatish uchun ishlatiladi. Aniqlash tizimi ichki sifat nazoratini o’z ichiga oladi, bu namunalarda PCR inhibitörleri mavjudligini yoki namunalardagi hujayralar olinganligini nazorat qilish uchun ishlatiladi, bu noto’g’ri salbiy vaziyatni samarali ravishda oldini oladi.

[ASOSIY KOMPONENTLAR]

To’plam 48 ta testni qayta ishlash yoki sifat nazorati uchun reagentlarni o’z ichiga oladi, shu jumladan quyidagi komponentlar:

Reagent A

Reaktiv B

Eslatma: Turli partiya raqamlarining komponentlarini bir-birining o’rnida ishlatib bo’lmaydi

[Saqlash shartlari va saqlash muddati] 
1.Reagent A/B 2-30°C haroratda saqlanishi mumkin, saqlash muddati esa 10 oy.
2.Please open the test tube cover only when you are ready for the test.
3.Yaroqlilik muddati o’tgan probirkalardan foydalanmang.
4.Oqishni aniqlash naychasidan foydalanmang.
[Qo’llaniladigan asbob]
LC480 PCR tahlil tizimi, Gentier 48E Avtomatik PCR tahlil tizimi, ABI7500 PCR tahlil tizimi uchun mos keladi.

[Namuna talablari]
1.Qo’llaniladigan namuna turlari: tomoq tayoqlari namunalari.
2. Namuna olish yechimi: After verification, it is recommended to use normal saline or Virus preservation tube produced by Hangzhou Testsea biology for sample collection.
throat swab: ikki tomonlama faringeal bodomsimon bezlarni va faringeal orqa devorni bir martalik steril namuna olish tamponi bilan artib oling, tamponni 3 ml namuna olish eritmasi bo’lgan naychaga botiring, dumini tashlang va trubka qopqog’ini mahkamlang.
3. Namunani saqlash va yetkazib berish: The samples to be tested should be tested as soon as possible. The transportation temperature should be kept at 2~8ºC.The samples that can be tested within 24 hours can be stored at 2ºC~8ºC and if the samples cannot be tested within 24 hours, it should be stored at less than or equal to -70ºC (if there is no storage condition of -70ºC, it can be stored at -20ºC temporarily), avoid repeated
muzlash va eritish.
4.To’g’ri namuna yig’ish, saqlash va tashish ushbu mahsulotning ishlashi uchun juda muhimdir.
[Sinov usuli]
1.Namunani qayta ishlash va namunani qo’shish
1.1 Namuna ishlash
Yuqoridagi namuna olish eritmasini namunalar bilan aralashtirgandan so’ng, 30 mkL namunani DNK ajratuvchi reagent trubasiga oling va uni teng ravishda aralashtiring.
1.2 yuklanmoqda
Take 20μL of the reconstitution reagent and add it to the MPV detection reagent, add 5μL of the above processed sample (The positive control and negative control shall be processed in parallel with the samples), cover the tube cap, centrifuge it at 2000rpm for 10 seconds.
2. PCR amplifikatsiyasi
2.1 Load the prepared PCR plate/tubes to the fluorescence PCR instrument, Negative control and positive control shall be set for each test.
2.2 Floresan kanal sozlamalari:
1) MPVni aniqlash uchun FAM kanalini tanlang;
2) Ichki nazorat genini aniqlash uchun HEX/VIC kanalini tanlang;
3.Results analysis
Set the base line above the highest point of the negative control’s fluorescent curve.
4.Sifat nazorati
4.1 Salbiy nazorat: FAM, HEX/VIC kanalida Ct qiymati aniqlanmadi yoki Ct>40;
4.2 Ijobiy nazorat: FAM, HEX/VIC kanalida, Ct≤40;
4.3 Yuqoridagi talablar bir xil tajribada bajarilishi kerak, aks holda sinov natijalari haqiqiy emas va tajribani takrorlash kerak.
[ Cut off value ]
Namuna quyidagi hollarda ijobiy deb hisoblanadi: maqsadli ketma-ketlik Ct≤40, ichki nazorat geni Ct≤40.
[ Results interpretation ]
Once the quality control is passed, users should check if there is an amplification curve for each sample in HEX/VIC channel, if there is and with Ct≤40, it indicated the internal control gene is successfully amplified and this particular test is valid. Users can proceed to the follow up analysis:
3.For samples with the amplification of internal control gene failed (HEX/VIC
kanal, Ct>40 yoki kuchaytirish egri chizig’i yo’q), kam Virusli yuk yoki PCR inhibitori mavjudligi muvaffaqiyatsizlikka sabab bo’lishi mumkin, tekshirish namunalar to’plamidan takrorlanishi kerak;
4.For positive samples and cultured virus, the results of internal control do not affect;
Salbiy sinovdan o’tgan namunalar uchun ichki nazorat ijobiy sinovdan o’tkazilishi kerak, aks holda umumiy natija noto’g’ri bo’ladi va namunani yig’ish bosqichidan boshlab tekshirishni takrorlash kerak.

[ Paket va transport ]

[ biz haqimizda ]