Kwayar cutar Monkeypox mai saurin gwajin kit 2022 MPV Nucleic Acid Detection Kit PCR Gwajin tare da Takaddar Yuro CE

[ GABATARWA ]
*Ana amfani da wannan kit ɗin don gano abubuwan da ake zargin sun kamu da cutar ta Monkeypox (MPV) da sauran cututtukan da * ke buƙatar bincikar kamuwa da cutar ta Monkeypox.
*Ana amfani da kit ɗin don gano ƙwayar f3L na MPV a cikin swabs na makogwaro da samfuran swab na hanci.
*Sakamakon gwajin wannan kit ɗin don nunin asibiti ne kawai kuma bai kamata a yi amfani da shi azaman ma’auni kaɗai don ganewar asibiti ba. An ba da shawarar yin nazari mai zurfi game da yanayin bisa ga bayyanar asibiti na marasa lafiya da sauran gwaje-gwajen dakin gwaje-gwaje. 

[FALALAR KYAUTA]

[Ka’ida]
Wannan kit ɗin yana ɗaukar takamaiman jerin abubuwan da aka adana na MPV f3L gene a matsayin yankin da aka yi niyya. Ana amfani da fasaha na PCR mai saurin haske na ainihin lokaci da fasahar sakin nucleic acid don saka idanu akan kwayar nucleic acid ta hanyar canjin siginar kyalli na samfuran haɓakawa. Tsarin ganowa ya haɗa da kulawar inganci na ciki, wanda ake amfani da shi don saka idanu ko akwai masu hana PCR a cikin samfurori ko kuma an dauki sel a cikin samfurori, wanda zai iya hana mummunan halin da ake ciki.

[MAFARKI KYAUTA]

Kit ɗin ya ƙunshi reagents don sarrafa gwaje-gwaje 48 ko sarrafa inganci, gami da abubuwan da ke biyowa:

Reagen A

Mai sake dubawa B

Lura: Abubuwan da aka haɗa na lambobi daban-daban ba za a iya amfani da su ba

[Yanayin Ajiya Da Rayuwar Shelf] 
1.Reagent A / B za a iya adana shi a 2-30 ° C, kuma rayuwar shiryayye shine watanni 10.
2.Da fatan za a buɗe murfin bututun gwajin kawai lokacin da kuke shirye don gwajin.
3.Kada ku yi amfani da bututun gwaji fiye da ranar karewa.
4.Kada a yi amfani da bututun ganowa mai zubewa.
[Kayan aiki mai dacewa]
Dace da dacewa da tsarin bincike na LC480 PCR, Gentier 48E Atomatik PCR tsarin bincike, ABI7500 PCR tsarin bincike.

[Sample Bukatun]
1.Amfani nau’in samfurin: samfurin swabs makogwaro.
2.Sampling Magani: Bayan tabbatarwa, ana ba da shawarar yin amfani da saline na yau da kullun ko bututun adana ƙwayar cuta wanda Hangzhou Testsea ilmin halitta ya samar don tarin samfura.
makogwaro: shafa tonsils na pharyngeal na biyu da bangon pharyngeal na baya tare da swab samfurin samfurin bakararre, nutsar da swab a cikin bututu mai ɗauke da maganin samfurin 3ml, jefar da wutsiya, sannan ƙara murfin bututu.
3.Sample ajiya da bayarwa: Ya kamata a gwada samfuran da za a gwada da wuri-wuri. Ya kamata a kiyaye yawan zafin jiki na sufuri a 2 ~ 8ºC. Ana iya adana samfurori da za a iya gwadawa a cikin sa’o’i 24 a 2ºC ~ 8ºC kuma idan ba za a iya gwada samfurori a cikin sa’o’i 24 ba, ya kamata a adana a ƙasa da ko daidai da -70ºC. (idan babu yanayin ajiya na -70ºC, ana iya adana shi a -20ºC na ɗan lokaci), guje wa maimaitawa.
daskarewa da narkewa.
4.Proper samfurin tarin, ajiya, da sufuri suna da mahimmanci ga aikin wannan samfurin.
[Hanyar Gwaji]
1.Sample aiki da samfurin ƙari
1.1 Samfurin aiki
Bayan haɗa maganin samfurin da ke sama tare da samfurori, ɗauki 30μL na samfurin a cikin bututun sakewa na DNA kuma haɗa shi daidai.
1.2 Loading
Dauki 20μL na reconstitution reagent kuma ƙara shi zuwa MPV gano reagent, ƙara 5μL na sama sarrafa samfurin (The tabbatacce iko da korau iko za a sarrafa a layi daya tare da samfurori), rufe tube hula, centrifuge shi a 2000rpm for 10 seconds.
2. PCR haɓakawa
2.1 Load da farantin PCR da aka shirya / bututu zuwa kayan aikin PCR mai haske, Ikon mara kyau da ingantaccen iko ya kamata a saita don kowane gwaji.
2.2 Saitin tashar mai walƙiya:
1) Zaɓi tashar FAM don gano MPV;
2) Zaɓi tashar HEX / VIC don gano kwayar cutar ta ciki;
3.Nazarin sakamako
Sanya layin tushe sama da madaidaicin madaidaicin madaidaicin lanƙwasa mai kyalli.
4.Kwafin inganci
4.1 Korau iko: Babu ƙimar Ct da aka gano a cikin FAM, tashar HEX / VIC, ko Ct> 40;
4.2 Kyakkyawan sarrafawa: A cikin FAM, tashar HEX / VIC, Ct≤40;
4.3 Abubuwan da ke sama yakamata a cika su a cikin gwaji iri ɗaya, in ba haka ba sakamakon gwajin ba shi da inganci kuma gwajin yana buƙatar maimaitawa.
[Yanke darajar]
Ana ɗaukar samfurin a matsayin tabbatacce lokacin: Jerin Target Ct≤40, Tsarin sarrafa ciki Ct≤40.
[ Fassarar Sakamako ]
Da zarar an ƙetare ingancin kulawa, masu amfani yakamata su bincika idan akwai madaidaicin haɓakawa ga kowane samfurin a cikin tashar HEX / VIC, idan akwai kuma tare da Ct≤40, yana nuna nasarar haɓakar ƙwayar sarrafawa ta ciki kuma wannan takamaiman gwajin yana da inganci. Masu amfani za su iya ci gaba zuwa bincike mai zuwa:
3.Don samfurori tare da haɓakawa na tsarin kulawa na ciki ya kasa (HEX / VIC
tashar, Ct> 40, ko babu ƙararrawa curve), ƙananan nauyin ƙwayar cuta ko kasancewar mai hana PCR zai iya zama dalilin rashin nasara, jarrabawar ya kamata a maimaita daga tarin samfurin;
4.Don samfurori masu kyau da ƙwayoyin cuta na al’ada, sakamakon kula da ciki ba ya tasiri;
Don samfuran da aka gwada ba su da kyau, ana buƙatar sarrafa iko na ciki don gwada inganci in ba haka ba sakamakon gabaɗayan ba daidai ba ne kuma ana buƙatar maimaita jarrabawar, farawa daga matakin tarin samfurin.

[ Shiryawa & Jigilar kaya ]

[ Game damu ]